An MBoC Favorite: Identification of novel principles of keratin filament turnover in living cells
نویسنده
چکیده
This study examines the attributes of keratin intermediate filaments in single, live, nonmotile epithelial cells under steady-state conditions. From their substantive findings, the authors proposed a spatially and temporally defined “keratin cycle” that accounts for the genesis, maturation, and turnover of keratin filaments in the setting of live epithelial cells in culture. I suspect that, just like me, many researchers did not expect that the process of assembly to turnover of keratin filaments would be so vectorial and spatially well-defined, even if it ultimately applies only to a cell culture setting. The “keratin cycle” also provided a conceptual framework from which to examine the relationships among keratin filaments, F-actin, microtubules, and adhesion complexes. The model has been refined since, and several of the predictions it originally generated have been upheld through subsequent experimentation. Finally, this study provided an alternative view of intermediate filament dynamics at steady state, complementing similar efforts from other researchers.
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Identification of novel principles of keratin filament network turnover in living cells.
It is generally assumed that turnover of the keratin filament system occurs by exchange of subunits along its entire length throughout the cytoplasm. We now present evidence that a circumscribed submembranous compartment is actually the main site for network replenishment. This conclusion is based on the following observations in living cells synthesizing fluorescent keratin polypeptides: 1) Sm...
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